CCP4 TUTORIAL PDF

Home · About CCP4 · CCP4 Projects · Downloads · Documentation · Courses · Developers · CCP4 people · WG1/WG2 · Privacy. Other MR examples can be found at the end of this tutorial, and at: When this tutorial is obtained as part of the CCP4 distribution, $MR_TUTORIAL. Previously Lecture Notes and Tutorial Material. 1、 Lecture Notes and Tutorial Material. iMosflm training · Mosflm examples. 2、 Lecture Notes and .

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Close all other interface windows except the main window.

This is given just before the “average spot profile” in the “mosflm. The masked area will come up as a red circle.

Wait while the task window re-draws itself. This is the best indicator of data quality.

CCP4 Tutorial – Session 2

The final tutorkal in spot positions should be one pixel or less. As established abovebatch number 74 is a rather serious outlier. See the accompanying document for an example where twinning occurs. The best way to locate the direct beam position is to look at a powder diffraction ring e. Molrep can make use of the target sequence to improve the search model.

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CCP4 Tutorial: Session 2 – Data Processing and Reduction

The output file has 49 columns:. The graphs in Acentric Moments of If this is less than 0. The maximum reflection width can be changed as follows: Remember to check adjacent images for partials.

The same hkl triplet may occur several times, with different instances being distinguished by different batch numbers. This depends on the mosaic spread and minimum separation.

If an incorrect format statement is given, the task fails with an error such as: If not, revert to the original value. By default all found spots from all images will be used in autoindexing, but any combination of images can be selected using Select images.

CCP4 Molecular Graphics – Tutorial Contents

Fractional bias may show an indication of “Partial bias”. Extra information is now displayed at the bottom of the display window. Use the cross-wires to estimate values.

When the refinement has finished, image 1 will be displayed with NO spots predicted and the message “Waiting for input” will appear over the image, and a summary of the refinement will be given in tutoriql output window:. If the mosaic spread is very high 1 degree or more a significant number of reflections will be flagged as “too wide in phi” green boxes. We will see later that all but the 0,0,0 peak are symmetry equivalents.

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These values should be a fraction of a pixel. Much of the information is the same as turorial the previous example, but there is some tutoriaal information specific to unmerged MTZ files. Programs use these names to identify the columns that are to be used. Finally you are given the option of selecting the solution default yes. These plots confirm there is no problem with twinning.

At the end of the tutorial, there are suggestions for web-based options. In such cases you should check that you supplied the correct beam coordinates, to avoid the risk of mis-indexing the image. Run as above, with the following differences:. The scaling will now be performed in space group H You also need to understand how information is arranged in an MTZ file. In this exercise, we tutoeial an unmerged MTZ file.