Introducción a la HPLC: aplicación y práctica. Front Cover. Oscar Alberto Quattrocchi. Artes Gráficas Farro, – pages. 6 oct. We are always happy to assist you. Document Description. Introduccion a La HPLC Quattrocchi. Document Share. Documents Related. Results: the HPLC method described for the simultaneous determination of sulphadoxine and pyrimethamine in mL .. Quattrocchi OA, De Andrizzi SA, Laba RF. Introducción a la HPLC Aplicación y. Práctica. Buenos Aires: Artes Gráficas.
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Each solution was applied as bands 3 introduccioh. Standards used were at concentrations of 0. Pharmaceutics, A carbon paste electrode was modified with the ionic liquid 1-methyloctyl imidazolium hexafluorophosphate and it was applied for study the electrocatalytic oxidation and voltammetric determination of the drug sulfamethazine.
Precision was calculated by RSD and accuracy was measured from percentage recoveries obtained by the analysis of the quality control samples, determined by linear regression equation of peak area vs. For determination of nifedipine in blood, some methods using HPLC, voltammetry and spectrophotometry 39,40 have been reported. Working solutions were prepared at concentrations of 0.
GentlemanCologne best place on net for men’s cologone. The best mobile phase was a mixture of chloroform: The method was sensitive enough to detect a quantity as low as 0.
Introduccion a La HPLC Quattrocchi
Three replicates each were prepared and each replicate was applied as bands three times. High performance thin layer chromatographic determination of nifedipine in human serum after liquid-liquid extraction. Therefore, the principal advantage of HPTLC is a fast analysis, with a high selectivity, accuracy, sensitivity nanograms in UV absorbance mode and picograms in fluorescence mode and precision.
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License. Care44 Solutions of concentration of 0. Application of the method The nifedipine concentration ranged between 0. Moreover, three independent blank human serum samples were checked for any interfering peaks.
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Chromatography Chromatography was carried out on HPTLC glass backed plates 10 x 10 cm and 20 x 10 cm precoated with silica gel Flayer thickness 0. The solutions were protected from light with an aluminum foil wrapping and usually freshly prepared solutions were used. Nifedipine also may be used in the management of hypertension in patients with low-renin hypertension as black patientscoexisting angina, peripheral vascular-disease, or isolated systolic hypertension, and in geriatric patients.
Hello GuiHn If you are begining with hplc and you understand spanish I recommend: Care37 Hashemi, Electroanalysis 24, Later, blank serum samples were spiked with this solution, to obtain final concentrations of 0.
Sample application was done on 4 mm bands. Cheng, Talanta81 Mon May 16, 8: The chromatographic conditions were arrived at after investigating several parameters, such as detection wavelength, ideal mobile phase and their proportions, internal standards, and optimal conditions for sample extraction.
The selectivity of the method was examined by preparing and analyzing checked by analyzing human serum samples spiked with nifedipine and the internal standard carbamazepine. Detection and quantification limits.
The mobile phase consisted of chloroform: Karimi, Talanta But, I will read this book too, thanks. Fri Jan 19, Application of the method. Quathrocchi wavelengths were tried, and nm was selected as working wavelength.
Resolution R S between both peaks was 3. The analysis is fast, and the method allows a large number of samples to be measured simultaneously. Precision and accuracy Quality control samples spiked at three concentrations 0.
Actuators B Latest Blog Posts from Separation Science. Quality control samples spiked at three concentrations 0. The results are presented in Tables 1 and 2. Med,